A simple blood test may detect HCC in its early stages, potentially improving patient outcomes

Topics: Featured
November 8, 2021

Liver cancer has the fastest growing incidence of any cancer in the United States and is a leading cause of cancer-related deaths worldwide, 1,2 with over 30,000 deaths anticipated in 2021 in the United States.3

Early detection of hepatocellular carcinoma (HCC) can increase survival rates significantly,4 and HCC surveillance is associated with increased early-stage detection and improved opportunity for curative treatment.5

There are, however, significant barriers to achieving widespread early detection. Adherence to recommended surveillance among at-risk patients remains low, and a recent systematic review estimated that fewer than 30% of patients received recommended surveillance.6

In addition, the recommended surveillance strategies have a relatively low and variable sensitivity for early-stage disease; the pooled average early-stage sensitivity for ultrasound and alpha-fetoprotein (AFP) is 63%, and for ultrasound alone, it is 47%.7

Over the last decade, liquid biopsies have emerged as a minimally invasive technique to improve early detection of multiple types of cancer.8,9 This approach is an alternative to surgical biopsy and involves sampling and analyzing an individual’s blood, urine, saliva, or other body fluid for evidence of informative biomarkers.8,10 For HCC, identifying tumor-specific biomarkers through bloodbased assays has shown promise.11,12

In the current issue of Clinical Gastroenterology and Hepatology, Chalasani et al report findings in a series of studies identifying and validating a new liquid biopsy assay for HCC surveillance, which is now commercially available as part of the Oncoguard® Liver solution.

This study used samples collected from an international, multicenter, case-controlled study to establish the multitarget HCC blood test algorithm and cutoff values and validate test performance (overall sensitivity, early stage sensitivity, and specificity) in patients who have chronic liver disease.12

The test is a simple yet sophisticated blood test for HCC using a combination of methylated DNA and protein biomarkers. It measures the DNA methylation markers HOXA1, TSPYL5, and B3GALT6 and the protein biomarker AFP and incorporates patient sex to classify samples as positive or negative for HCC.12

The algorithm development portion of the study consisted of 136 HCC cases (60% early stage) and 404 controls. Study subjects had underlying cirrhosis or chronic hepatitis B virus.12 In the validation portion of the study, the test performance was clinically validated in an independent cohort of 156 HCC cases (50% early stage) and 245 controls. The results were consistent and showed 88% overall sensitivity, 82% early-stage sensitivity, and 87% specificity,12 thereby enabling an accurate identification of patients with or without HCC.

Additional analyses comparing the test to currently available surveillance tools were also conducted. The multitarget HCC blood test showed greater early-stage sensitivity than that of AFP and GALAD. The results indicated that sensitivity of the test (82%) was significantly higher than both AFP (40% at a cutoff of 20 ng/mL, P<.001) and GALAD (71% at a cutoff of -0.63, P=.03) for patients who had early-stage HCC. The test also demonstrated enhanced sensitivity (81%) for patients within the Milan criteria (ie, a single tumor ≤5 cm or up to three nodules ≤3 cm without macrovascular invasion or extrahepatic spreading), which was higher than an AFP level of 20 ng/mL or greater (41%; P<.0001) but not significantly higher than a GALAD score of -0.63 or greater (71%; P=.07).12

The authors concluded that, with its greater early-stage sensitivity, versus guideline-recommended blood tests, the multitarget HCC blood test may significantly improve early-stage HCC detection for at-risk patients undergoing HCC surveillance, which is a critical step toward increasing curative treatment opportunities and reducing mortality.12

The study by Chalasani et al in this issue of Clinical Gastroenterology and Hepatology provides important new data from contemporary research that adds to the evidence base supporting the use of biomarkers and the multitarget HCC blood test for early HCC detection.12

This new multitarget HCC blood test may empower healthcare providers with a tool that is accurate and has a broad application in clinical practice. For patients, family members and caregivers, it offers a convenient route to testing that may bypass some of the logistical challenges often associated with traditional surveillance methods.13 Ultimately, for patients, it offers the potential for early detection of HCC, so the goal of achieving long-term survival may be within reach.

Looking ahead

We envision a future where physicians and their patients are educated about the early detection of HCC and supported with simple and convenient administrative tools that make routine HCC surveillance simpler. To achieve this vision, we’ve developed a highly sensitive test that enables early-stage detection of HCC, thereby giving patients the chance to undergo potentially curative treatments.

Stay tuned and watch for future blogs that will take a look at the prevention of HCC and the latest research about HCC and its surveillance.

Visit us again soon at OncoguardLiver.com/education to learn more about the future of HCC surveillance.

The foregoing information is for informational purposes only and is not treatment advice for any patient. Physicians should use their clinical judgment and experience when deciding how to diagnose or treat patients.


Henley SJ, Ward EM, Scott S, et al. Annual report to the nation on the status of cancer, part I: national cancer statistics. Cancer. 2020;126(10):2225-2249. doi: 10.1002/cncr.32802

Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021. doi: 10.3322/caac.21660

Siegel RL, Miller KD, Fuchs HE, et al. Cancer statistics, 2021. CA Cancer J Clin. 2021;71:7-33.

American Cancer Society. Cancer facts & figures 2021. Published January 2021. https://www.cancer.org/research/cancer-facts-statistics/all-cancer-facts-figures/cancer-facts-figures-2021.html. Accessed February 1, 2021.

Singal AG, Pillai A, Tiro J. Early detection, curative treatment, and survival rates for hepatocellular carcinoma surveillance in patients with cirrhosis: a meta-analysis. PLoS Med. 2014;11(4):e1001624. doi: 10.1371/ journal.pmed.1001624

Wolf E, Rich NE, Marrero JA, Parikh ND, Singal AG. Use of hepatocellular carcinoma surveillance in patients with cirrhosis: a systematic review and meta-analysis. Hepatology. 2020. doi: 10.1002/hep.31309.

Tzartzeva K, Obi J, Rich NE, et al. Surveillance imaging and alpha fetoprotein for early detection of hepatocellular carcinoma in patients with cirrhosis: a meta-analysis. Gastroenterology. 2018;154:1706- 1718.

Dominguez-Vigil IG, Moreno-Martinez AK, Wang JY, Roehrl MHA, Barrera-Saldana HA. The dawn of the liquid biopsy in the fight against cancer. Oncotarget. 2018;9(2):2912-2922. doi: 10.18632/ oncotarget.23131

Cree IA, Uttley L, Buckley Woods H, et al. The evidence base for circulating tumour DNA blood-based biomarkers for the early detection of cancer: a systematic mapping review. BMC Cancer. 2017;17(1):697. doi:10.1186/s12885-017-3693-7

Campos CDM, Jackson JM, Witek MA, Soper SA. Molecular profiling of liquid biopsy samples for precision medicine. Cancer J. 2018;24(2):93- 103. doi: 10.1097/PPO.0000000000000311

Chalasani NP, Ramasubramanian TS, Bhattacharya A, et al. A Novel Blood-Based Panel of Methylated DNA and Protein Markers for Detection of Early-Stage Hepatocellular Carcinoma. Clin Gastroenterol Hepatol. 2020:S1542-3565(1520)31224-31226. doi: 10.1016/j. cgh.2020.08.065

Chalasani NP, Porter K, Bhattacharya A, et al. Validation of a novel multitarget blood test shows high sensitivity to detect early stage hepatocellular carcinoma. Clin Gastroenterol Hepatol. 2021. Published August 12, 2021. doi: 10.1016/j.cgh.2021.08.010

Farvardin S, Patel J, Khambaty M, et al. Patient-reported barriers are associated with lower hepatocellular carcinoma surveillance rates in patients with cirrhosis. Hepatology. 2017;65(3):875-884. doi: 10.1002/hep.28770